The covalent binding of radiolabeled 2-aminomethylimidazo[4,5-f]quinoline (IQ) and 2-acetylaminofluorene (AAF) to cell macromolecules was studied using primary monolayer cultures of adult rat hepatocytes. A time course for covalent binding was determined, and revealed similar levels of binding for both chemicals. Cell Isolation and Culture. Hepatocytes from adult male Sprague-Dawley rats, weighing g, were isolated by a two-step collagenase perfusion procedure as described previously (Guguen et al., ).Hepatocytes were seeded at × 10 4 cells/cm 2 on plastic dishes or cm 2 flasks for total RNA preparation, in a mixture of 75% minimum essential medium and 25% .
Abstract We investigated the effects of α- and β-adrenergic agonists on epidermal growth factor (EGF)-stimulated extracellular-signal regulated kinase (ERK) isoforms in primary cultures of adult rat hepatocytes. Hepatocytes were isolated and cultured with EGF (20 ng/ml) and/or α (1)-, α (2)- and β (2)-adrenergic agonists. Adult rat hepatocytes underwent significant DNA synthesis after culture with 5 ng/ml HGF for 3 h at a low cell density ( × 10 4 cells/cm 2). Under these culture conditions, the number of nuclei increased significantly during a subsequent 4-h culture period.
I. Evidence that glucocorticoids and pregnenolone 16α-carbonitrile regulate de novo synthesis of a common form of cytochrome P in cultures of adult rat hepatocytes and in the liver in vivo, J. Biol. Chem. – PubMed Google Scholar. Intrasplenic HTX of 5 × 10 7 reversibly immortalized human hepatocytes, which are equivalent to about 5% of the total number of hepatocytes per adult rat, was able to protect animals from ALF. Why could such a small number of hepatocytes enable the rats to recover from ALF?